The Real Gumbo Recipe

I wanted to follow up requests in response to my post on Spices, and share the gumbo recipe I mentioned. 

It’s my mom’s from her mom, Nannie (Louise P. Curtis)

I’ve used Nannie’s original language

Start with 1 large TBS bacon, ham or sausage drippings in heavy saucepan

Chop 1 pound of okra, 1 large onion, 1 large bell pepper and 5 cloves of garlic

Fry in oil until wilted. Okra will lose stringiness.

Add 1-2 TBS of flour. Cook slowly about 30-45 minutes until okra gets drier.

Add 1 can tomato paste

Add hot seafood water, about 4 cups

Add seafood 1  lb crabmeat and 1 lb peeled shrimp

Add seasonings:

  • 2 drops Tabasco or cayenne pepper
  • salt and pepper to taste
  • a couple of bay leaves
  • chopped celery (1/4 cup)
  • green onions and parsley (optional)

Simmer for about 1 hour over very low fire

Serve over rice


Note: I always added olive oil and Louisiana Crystal Hot Sauce and opened a bottle of Jax or two.



I was lucky enough to grow up all over the world – Thailand, Hong Kong, Germany, Romania and Greece. Some of my fondest memories are around food. My mom was a fabulous cook, which was a vital part of my father’s work as a cultural affairs officer in the US Foreign Service.

Vanilla bean plant

One of my earliest memories was of making a landing in Anchorage Alaska on a return trip from Bangkok to my parents’ home town of Biloxi, Mississippi. I remember the large stuffed polar bear in the lobby, and more importantly, my first taste of vanilla ice cream. Having come from the heat of Bangkok, with my daily food rich in hot peppers and ginger, this cooling fragrant treat was nectar from the gods.

When I started to learn to cook standing over my mom’s pot of gumbo she stressed the importance of simmer with the last ingredients – two bay leaves. They looked so simple – small dried plant material –  but an hour later, the aroma of my Nannie’s gumbo was bigger than just the file and seafood, with the bay leaves providing a vibrant top note.  Years later, deep into a career researching active molecules in medicinal plants, I realized that the food culture I grew up in was full of medicinal plants in the spices added to our evening meals.

Bay Leaf

Spices both sense of taste and smell. Our sense of smell, in particular, is influenced by the characteristics of the molecules responsible for odor. How quickly do they volatilize (how easily molecules vaporize)? How soluble are they in water or oil?  How acidic is the food (a tomato based food or something with lemon)? Even our genes can influence the sense of smell.

A molecule that we can sense through smell is called a fragrance or odorant. Those molecules need to reach the olfactory system in the upper part of our nose. To do so, generally the molecular weight of these molecules needs to be equal to or less than 300 g/mol (a scientific designation for the mass of a substance divided by the amount of the substance). Ultimately, our sense of smell comes down to a pattern of activity of neurons in the brain responding to the stimulus of odor molecules binding to receptors (Malnic et al., 1999).

We have receptors (a protein in a cell membrane that responds to molecule attaching to it) in our nasal passages that are triggered by these molecules. Each olfactory receptor recognizes more than one odorant, and each odorant can be detected by several different olfactory receptors. This reflects a combinatorial process common to biological systems. Several factors influence the resulting signal sent from the receptor site.

We know that the shape of a molecule is important (Saberi and Seyed-Allaei, 2016), and that as the molecule binds (or sticks to) a receptor, the receptor changes shape, which leads to neural signals reaching the brain.  Several theories exist to help us understand how to map the process of detecting odors, based largely on chemical qualities of the odorants.

Mori et al. (1994) suggested that the odor signal are more complex than a single receptor binding event, rather the signal is a product of a series of receptor excitations from numerous receptor sub sites (odotypes). This became known as the Odotope Theory. This theory also explains odor-less molecules as the presence sub sites more numerous than the limit of potential binding sites.

A second important theory, Dyson (1938) originated the Vibrational Theory that Wright (1982) later refined. This theory posits that olfactory receptors might really sense vibrational energy on a quantum level rather than structural shapes of the molecule when detecting odors. Quantum mechanics describes nature at the smallest scales of energy levels of atoms or substances.

Combining the two theories, Turin and Yoshi suggest that the tightness of receptor binding, based on both the physical and charge shape (polarity) of an odorant molecule may influence the intensity of the odor, while the character of the odor is effected by vibrational characteristics of the molecule.

To help visualize the theories, we can take a series of molecules found in medicinal plants that have a common base structure, and explore how their odor characteristics may be influenced by chemical features such as solubility, volatility, molecular shape and size (see Table 1).

Starting with the basic structure, vanillin give vanilla its signature sweet, perfumed, woody aroma. The molecular weight is relatively low, and it volatilizes easily, filling a room with the odor when cooked. The oxygen R-groups (groups that “hang” from the ring) on the benzene ring of vanillin make it highly solubility in water.

Built from the same basic structure, eugenol has a short hydro-carbon tail that gives it a stronger odor than vanillin, the familiar aroma found in bay leaf or allspice. This hydro-carbon tail also makes it more fat soluble and may influence the intensity of receptor binding. The odor threshold of eugenol is also lower than that of vanillin. The less polar binding site on the molecule may influence the strength of binding in this example, and explain why the vanillin odor is not as persistent as that of bay leaf or allspice.

Table 1: Vanillin Based Molecular Structures with Odor Thresholds


(Odor Threshold: 20-200ppb)*


(Odor Threshold: 6-30ppb)


(Odor Threshold: na)


(Odor Threshold: na)


Ginger plant

Zingerone has an even longer hydro-carbon tail connected to the basic vanillin shape, making it insoluble in water. Found in ginger and mustard oil, it gives a rich, sweet, warm and woody fragrance. However, the presence of the carbonyl group (C=O) in the tail means that the zingerone molecules tend to attract each other, limiting how easily it volatilizes. Thus, ginger is less likely to fill a room as quickly with its aroma than vanilla.

Even though Capsaicin also has a long, hyrdo-carbon rich tail, the polar amide group (-NHCO-) makes it slightly more soluble in water than zingerone. The size of the tail also limits the molecule’s volatility. Capsaicin has no odor, and given the numerous sites along that long tail with the potential of binding to a receptor sub sites, this may be an example of a molecule with too many binding sites, as the Odotope theory suggests.

Chili peppers

So the next time in you are in the kitchen and the aromas are filling your senses, see if you can think about the shapes and characteristics of the molecules influencing that wonderful moment.



  1. Malnic B, Hirono J, Sato T, Buck LB. (1999) Combinatorial receptor codes for odors. 96(5), 713.
  2. Saberi M, Seyed-Allaei H (April 2016). “Odorant receptors of Drosophila are sensitive to the molecular volume of odorants”. Scientific Reports. 6: 25103. doi:1038/srep25103.
  3. Mori, K. and Shepherd, GM. (1994). Emerging principles of molecular signal processing by mitral/tufted cells in the olfactory bulb. Semin Cell Biol 5-1:65-74.
  4. Dyson, G.M. The scientific basis of odour . Chem Ind 57: 647-651.
  5. Wright, R.H. (1982) The sense of smell CRCpress, Boca Raton, Florida, USA.
  6. Turin, L, and Yoshi, F. (2003) Structure-Odor Relations: a modern perspective in Handbook of Olfaction and Gustation Second Edition. Doty RL, Ed. New York Marcel Dekker.




Sister Morphine

Although I’ve met and friended folks who claim they’ve seen it, I’ve never witnessed a plant that could run. Instead, they engage in chemical warfare or communication. The chemicals are the result of multi-step metabolic networks that provide the chemical apparatus to change the staring material into a bioactive substance. Such a chain of chemical reactions is controlled by a series of proteins, called enzymes. Each protein is coded for by a gene.Poster_papaver_3a

This research identified a large cluster of 15 genes that encode enzymes in the metabolic pathway with morphine as an endpoint. Approximately 50 alkaloids are found in Opium poppy (Papaver somniferum), with morphine the largest in concentration.

According to the study, the pathway for the painkilling drugs evolved around 7.8 million years ago (mya). Primates are presumed to have appeared 63 mya, Hominidae (precursors to modern humans) 15 mya, and humans 1.3-1.8 mya. First recorded humans use appears in 5000 BCE in the Neolithic age. The PBS show Frontline provides a timeline of human use. It’s history shows that Morphine has been a “wonder” drug for pain, and a bane for those addicted to it and it’s derivatives

The mechanism responsible for euphoria also kills. Morphine binds to receptors in the brain, inhibiting neurotransmitter release and resulting in among other physiological changes, pain relief, but also slowed breathing.  Overdose victims often stop breathing.

Since the plant has been around for quite some time, I wondered if there were any histories to show animals consuming this or other plants to reduce pain or to  give pleasure? A brief review showed the following:

  • Researchers discovered it was chili peppers. Next, they studied tree shrews in the wild and discovered they ate one particular pepper, the Piper boehmeriaefolium, and actually preferred to eat it over other plants and vegetation.
  • Scientific Reports provides evidence that Borneo based apes chew leaves of the Dracaena cantleyi plant to create a white lather, which they then rub onto to their bodies
  • A study of chimps found that they roll Aspilia leaves for a period of time (they are very bitter to chew). This plant material contain thiarubine A , which kills harmful bacteria, and fungi because they contain thiarubine A, a powerful antibiotic. Research also suggests these leaves act as a stimulant, since chimps ingest them first thing in the morning.

What’s more compelling is the rich association of the opium poppy with war.  with a few examples below. One aftermath of each – the trail of addiction that followed either the imposition of trade or the use of morphine on the battlefield to reduce pain from horrible damage.

  • During the 18th century, forcibly exported opium to China, even while it was banned in Britain because the government and industry knew it was not good for the populace in general. It took two opium wars, eventually disrupting the country and leading to the  collapse of the Qing Dynasty.
  • After World War I in remembrance of the fallen soldiers, the living commemorate the sight of thousands of blood-red poppies appearing on the battle-scarred fields of Flanders, in Northern France.
  • US wars in Vietnam and Afghanistan have been greatly affected by opium production supporting the opposing militaries ability to pay for the fighting.

Now America is facing a public health crisis of opioid addiction.  In an interesting turn, last November, President Donald Trump asked Chinese President Xi Jinping to help stop the “flood of cheap and deadly” fentanyl from China into the United States.  Fentanyl is a synthetic opioid, 50 to 100 times stronger than morphine.

For better or worse, this is an example of co-evolution – humans identifying and applying a plant to alleviate the pain they, themselves create.

Unique Germination Characteristics of Goldenseal Seed

goldensealUnderstanding dormancy requirements for woodland, medicinal plant species is a requirement for discovering how they initiate their relationship with soil fungi. Sanders & McGraw (2002) noted that despite wide geographic distribution, seedling establishment is a constraint in wild goldenseal (Hydrastis canadensis, L.) populations. Richo Cech, of Horizon Herbs, has been quite successful teasing recalcitrant, deep-forest medicinal species to break dormancy using forest propagation studies (2002). Baskin and Baskin (2014) noting that goldenseal was a two-phase germinator, with some seed germinating only as root tissue and lacking aerial development until the second year. They indicated that this appeared to be a developmental pattern found in several native plant species growing in the same habitat as goldenseal.

From the stand point of understanding the chemical ecology of medicinal plants, this may allow the roots to interact with the rhizosphere community of fungi longer and, in the case of goldenseal, to develop the anti-microbial alkaloid pool necessary for defense of aerial growth the following season. In a closely related plant family, Berberidaceae, alkaloid production in Berberis vulgaris occurs immediately after seed germination and increases with seedling age (Pitea et al. 1972). This suggests that the medicinally active alkaloids in root tissue are important to the defense of the plant seedling from the onset.

Data is lacking on goldenseal rootlet interactions with rhizosphere fungi post-germination. Initial screenings of mature, wild goldenseal root populations (Tims, 2008) indicated that arbuscular mycorrhizal fungal (AMF) were not associated with either root or seed tissue, and that AMF spores were not found in the rhizosphere soil of the plant. Goldenseal roots did appear to form an endophytic relationship with a zearalenone (ZON) producing Fusarium oxysporum, normally associated with pathogenic characteristics (Tims and Bautista, 2007).

Zearalenone (ZON) has reputed auxin-like (plant hormone) effects on plant tissue, promoting development of lateral roots (Celenza et al. 1995), and stimulating root tip growth (Bean et al. unpublished). These are regions of the root where soil fungi would attempt to enter the plant tissue. In contrast, this particular endophyte appeared to co-exist within the plant root without causing obvious signs of ill health. It is possible that the production of ZON by Fusarium may affect meristematic activity in H. canadensis emerging from dormancy by initially stimulating rootlet formation and root exudation. Rootlet interaction with ZON during the early phase may benefit the plantlet by increasing plant secondary metabolite formation.

Tims and Bautista found that an alkaloid in mature goldenseal root tissue, hydrastine, inhibited ZON production in the endophyte. Cech (private communication) collected cultivated goldenseal from Kentucky and Oregon, and found that berberine levels were higher in the leaf and hydrastine levels higher in the root.  This too would corroborate the results by Shitan et al. (2005) that an ABC pump moved berberine from root tissue to aerial portions of the plant. The interplay between ZON produced by the fungus and hydrastine found in goldenseal root would appear to  have a two-fold effect. Hydrastine may limit more aggressive pathogenesis of the root tissue by F. oxysporum, while ZON may stimulate the production of hydrastine in developing goldenseal root. An additional developmental question that needs to be explored is to what degree might the presence of ZON, or similar microbial compounds in the rhizosphere, favor or induce two-phase germination?


  1. Bean, GA. (1999) Unpublished.
  2. Baskin, CC and Baskin, JM. (2014) Seeds: Ecology, Biogeography, and, Evolution of Dormancy and Germination, 2nd Edition. Academic Press: Cambridge, MA.
  3. Cech, R. (2002) Growing At-Risk Medicinal Herbs: Cultivation, Conservation, and Ecology. Horizon Herbs Publication, Williams, Oregon, pp.41-51.
  4. Celenza JL, Grisafi PL, Fink GR (1995) A pathway for lateral root formation in Arabidopsis thaliana. Gene Dev 9: 2131–2142.
  5. Pitea, M, and Margineanu, C. (1972) Correlations between Chemical Structure and Antibacterial Activity of Berberine. Clujul Med 45:465.
  6. Sanders, SM, and McGraw, JB. (2002) Distribution, Abundance, and Population Dynamics of Goldenseal (Hydrastis canadensis L.) in an Indiana Nature Preserve, USA. Nat Areas J 22:129.
  7. Shitan, N, Kiuchi, F, Sato, F, Yazaki, K, and Yoshimatsu, K. (2005) Establishment of Rhizobium- Mediated Transformation of Coptis japonica and Molecular Analyses of Transgenic Plants. Plant Biotech 22:113.
  8. Tims, M.C. (2008) The Chemical Ecology of Goldenseal (Hydrastis canadensis L., Ranunculaceae): How medicinal plants roots control the microbial communities in their rhizosphere. VDM Verlag, Saarbracken Germany.
  9. Tims M.C. and Bautista C., (2007) Effects of Root Isoquinoline Alkaloids from Hydrastis canadensis on Fusarium oxysporum isolated from Hydrastis Root Tissue, Journal of Chemical Ecology, 33:1449–1455.

Co-Evolution of Humans and Plants

How do you interact with plants? ? Why do you interact with plants?

brussel sprouts

We respond to a spectrum of sensory effects

  • visual – pigments
  • taste – spice
  • smell – aromatic oils
  • effect – pharmacologically active

Evolutionarily speaking, it remains unclear whether pharmacological use of plants by humans was more prevalent before or after the development of agriculture led to cultivars with reduced biological activity compared to the wild types. Dr. Fatimah Jackson, at the University of Maryland, College Park, argues succinctly that cultural evolution – driven by language – became the driver influencing the extent of human interaction with plants. Dietary preferences are central to how cultures self identify and define. According to Daniel Moerman at the University of Michigan, Native Americans used plants in a 5:1 ratio as medicine and food. Over time humans have learned how to limit their exposure to toxic plants. I imagine a group of early humans going out as a group and asking ‘Mikey’ to try the plant first. If he lived, ‘Mikey’ discovered how to modifying plants’ palatability, nutrition, toxins and to amplify beneficial effects through various means – extraction, heating, drying, and fermentation to name a few. What examples exist from your own cultural heritage of unique use of plants and their chemistry?

Is this a form of co-evolutionary symbiosis between humans and plants? I would argue that humans have had profound effect on the genotype and phenotype of cultivated plants, while plants have provided nutrition, medicine, and the early stimulus for our enzymatic detoxification system and possibly for language development in the brain (synaesthesia – discussed in a future post).  Dietary exposure to continuous low levels of plant mutagens would certainly effect mutation rates or genetic drift. I would highly recommend an article by Dr. Jackson on human-plant-parasite triads as evidence for coevolution.

Consider the next you avoid eating your bitter tasting brussel sprouts – if you don’t eat them, are you de-evolving?